thr77 login Options
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been noticed in several tumors, which leads to chromosome instability, and so may possibly lead to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant job in endorsing cell-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are actually described. Two additional splice variants ended up documented but detailed nucleotide sequence information and facts is just not yet readily available.
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Blocking peptides are peptides that bind specifically to the goal antibody and block antibody binding. These peptide normally has the epitope regarded via the antibody.
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This guideline outlines further more feasible optimization of cellular and immunoassay parameters to make certain the absolute best outcomes are...
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This guideline outlines further doable optimization of mobile and immunoassay parameters to ensure the best possible final results are...
Antibodies certain to the blocking peptide not bind on the epitope about the goal protein. By evaluating the staining through the blocked antibody as opposed to the antibody alone, you can see which staining is click here precise.
Focus on Data Cyclin E belongs for the very conserved cyclin relatives, whose customers are characterized by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins operate as regulators of CDK kinases. Various cyclins exhibit unique expression and degradation styles which add for the temporal coordination of each and every mitotic party. Cyclin E forms a fancy with and functions like a regulatory subunit of CDK2, whose exercise is necessary for mobile cycle G1/S changeover. This protein accumulates in the G1-S stage boundary and is degraded as cells development by way of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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